Estación Fitopatolóxica do Areeiro Web Site - www.efa-dip.org - Papers 2003

		UNIDAD ASOCIADA "SISTEMAS AGROFORESTALES": ESTACIÓN FITOPATOLÓXICA DO AREEIRO - MISIÓN BIOLÓGICA DE GALICIA

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	XXX Congreso Nacional de Parques y Jardines, La Coruña 2003

		Camelias en el Castillo de Soutomaior
		Principales patógenos identificados sobre camelia japónica en los parques y jardines de Galicia

	IX Congreso Nacional de Ciencias Hortícolas, Pontevedra 2003

		Principlaes virosis detectadas en los cultivos hortícolas de la comunidad gallega
		Patógenos fúngicos, asociados a enfermendades degenerativas de madera, aislados en viñedos de la comunidad gallega
		Ácaros fitoseidos sobre cultivos hortícolas en la comarca de O Salnés: primeros resultados
		Influencia de la aplicación de fitorreguladores en el patrón de desarrollo y acumulación estacional de carbohidratos en el fruto de kiwi, en Pontevedra
		Seguimiento del ciclo productivo completo de diferentes varidades de mora en el noroeste de España
		Efecto de la inoculación de Glomus aggregatum en la producción de plantas de tres patrones de vid en vivero

	IXXVI Congreso SEBBM (Sociedad Española de Bioquímica y Biología Molecular), La Coruña 2003

		Comparación de tres protocolos par la extracción de ADN a partir de diferente material vegetal de Camellia

XXVI Congreso SEBBM. La Coruña 2003

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Comparación de tres protocolos para la extracción de adn a partir de diferente material vegetal de Camellia

Vela, P.; Pais, M.; Aguín, O.; Salinero, M.C.

Excma Diputación Provincial de Pontevedra. Servicio Agrario. Estación Fitopatolóxica do Areeiro. Subida a la Robleda s/n 361533 Pontevedra pmansilla@efa-dip.org

ABSTRACT: Camellia genus is abundant in Galicia, and can be seen both in public and private gardens. The identification of Camellia species using traditional methods such as agromorphological characters of the leaf and flower, shrub size, etc has been difficult, mainly due to the ease of hybridization and introgression. This factor may be influenced by onthgenic and environmental factors, which hide the real genetic diversity. Those methods based on the analysis of the genetic material may be an efficient tool for species, hybrids and cultivars determination. As it in happens for other woody species, one problem will be the difficult to obtain an optimum DNA, due to the presence of certain elements that could interfere in the extraction process. Thus, the aim of this work was to determine the type of Camellia plant material and the type of extraction process which will allow us to obtain in a rapid and efficient way, DNA to perform later molecular studies. Plants from 19 Camellia species cultivated in Galicia were selected, and from each of them: buds, shoots, leaves, flower buds and flowers were collected. Before the extraction, plant material was differently preserved: 1/3 was frozen, 1/3 lyophilized and 1/3 was kept as fresh. 3 extraction protocols were used with all of them. Results showed that the best material to perform DNA extraction were flower buds and buds (fresh, frozen and lyophilized) using the protocol of the Puregene DNA isolation Kit.