UNIDAD ASOCIADA "SISTEMAS AGROFORESTALES": ESTACIÓN FITOPATOLÓXICA DO AREEIRO - MISIÓN BIOLÓGICA DE GALICIA
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UNIDAD ASOCIADA "SISTEMAS AGROFORESTALES": ESTACIÓN FITOPATOLÓXICA DO AREEIRO - MISIÓN BIOLÓGICA DE GALICIA |
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| Home » Publications » Papers » 2005 |
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VI Congreso Forestal
Español, Zaragoza 2005
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Aplicación del método de conversión para la obtención de cepas hipovirulentas de Cryphonectria parasitica Aguín Casal, O.; Montenegro Gregorio, D.; Pérez Otero, R.; Mansilla Vázquez, J. P. Estación Fitopatolóxica do Areeiro. Servicio Agrario. Excma. Diputación Provincial de Pontevedra. Subida a la Robleda s/n.36153 Pontevedra. efa@efa-dip.org |
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ABSTRACT: Cryphonectria parasitica is a quarantine pathogen that is causing important damage on chestnuts in NW Spain. Biological control based on hypovirulence has proved to be efficient in reducing the incidence of the disease. In order to implement it, it is of crucial importance to determine the diversity and distribution of C. parasitica populations, and obtain hypovirulent strains compatible with compatibility types occurring in the area affected. The detection of hypovirulent strains, which is based on morphological and molecular criteria, have proved to be difficult, and in case they are found, unfortunately, they may be incompatible with established compatibility types. In the present work, the results obtained in the compatibility test among virulent strains obtained in several areas in Spain with C. parasitica hypovirulent strains detected in other countries, are presented in order to determine their conversion ability. These pairings are kept under the light at 25 ºC and are checked 11, 13 and 20 days later. Conversion from virulent to hypovirulent is confirmed in subcultures and dsRN extraction. Keywords: chestnut blight, compatibility, biological control, dsRNA |
