Consejo Superior de Investigaciones Científicas
UNIDAD ASOCIADA "SISTEMAS AGROFORESTALES": ESTACIÓN FITOPATOLÓXICA DO AREEIRO - MISIÓN BIOLÓGICA DE GALICIA
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Home » Publications » Papers » 2007

Reunión Científica del Grupo de Trabajo de Sanidad Forestal de La Sociedad Española de Ciencias Forestales
Palencia, 25 - 26 de September 2007

27


Variaciones en la susceptibilidad a Phytophthora cinnamomi de diferentes clones de castaño: comparación de dos métodos de inoculación


RIAL, C.¹, SALINERO, C.¹; MANSILLA, J.P. 1,2; PINTOS, C.¹ Y CUENCA, B. ³

¹Estación Fitopatolóxica do Areeiro. Excma. Diputación Provincial de Pontevedra. Subida a la Robleda s/n. 36153 Pontevedra. efa@efa-dip.org

²Departamento de Producción Vegetal, Universidad de Santiago de Compostela, Campus Universitario, 27002 Lugo, España.

³TRAGSA. Vivero de Ourense. Departamento de Mejora Agroforestal. Crta. Maceda-Valdrey km. 2. 32708 Maceda, Orense. bcuenca@tragsa.es




ABSTRACT: The study aims to determine the tolerance degree of several chestnut hybrid species and clones to P. cinnamomi. Thus, a comparative assay was carried out in which C. sativa and C. mollisima and Euro hybrid Asiatic clones, both provided by the TRAGSA nursery in Ourense, were studied in two development stages - acclimatized plants and explants. Acclimatized plants were acclimatized in the Estación Fitopatolóxica do Areeiro chambers and were later inoculated with growing P. cinnamomi mycelium, which was added to the substrate surface by diffusion. Explants, once propagated and in vitro rooted, were inoculated with mycelium pieces containing fungus sporangia. Plants were analyzed to check pathogen reisolation, a process performed at the EFA laboratories.
Susceptibility differences were observed among species and chestnut clones, as well as between both inoculation methods, finding that the hybrid clone HS and C. mollissima were the less susceptible to P. cinnamomi attack..


Keywords: "in vitro", explants, clon HS, C. mollisima