UNIDAD ASOCIADA "SISTEMAS AGROFORESTALES": ESTACIÓN FITOPATOLÓXICA DO AREEIRO - MISIÓN BIOLÓGICA DE GALICIA
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UNIDAD ASOCIADA "SISTEMAS AGROFORESTALES": ESTACIÓN FITOPATOLÓXICA DO AREEIRO - MISIÓN BIOLÓGICA DE GALICIA |
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| Home » Publications » Papers » 2010 |
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XV congreso de la
Sociedad Española de Fitopatología Vitoria 27 September - 1 October, 2010
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| Identification of Fusarium species transmitted by insect vectors in conifers growing in Galicia Aguín, O., Escofet, P., Pérez, R., Mansilla, P Estación Fitopatolóxica do Areeiro. Deputación de Pontevedra. Subida a la Robleda s/n, E-36153 Pontevedra, Spain. |
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ABSTRACT: The genus Fusarium includes fungi causing damages on a wide range of plant species. Among the most important plant pathogenic species of this genus we can include F. circinatum (quarantine pathogen), agent of pine pitch canker, that causes important economic losses in conifer production and Fusarium proliferatum and Fusarium verticillioides that are not quarantine pathogens, but that may cause damages in seeds and pine seedlings. A great variety of insects may act as vectors of species of this genus. The aim of the present work was to determine the insects involved in the transmission of Fusarium spp. in Galicia. From 2008 to 2009, 3,000 insects collected in 12 funnel Lindgren traps and Theysohn traps were analyzed. These traps were placed in Pinus pinaster and Pinus radiate stands in the four Galician provinces. The detection of Fusarium spp. was performed by placing the insects in specific culture media. The identification of Fusarium species was carried out by observing the morphological features of the colonies and by molecular analysis. For the molecular study, two regions of the fungal DNA were amplified: the intergen region of ribosomal DNA (ITS) and a region of the nuclear gene that codifies for the elongation factor (EF). The detection and identification of F. circinatum was performed directly from the insects without previous fungal isolation in the culture. To obtain F. circinatum DNA, insects were washed with an extraction buffer and the amplification of the extracted DNA was performed following two proceedings of real-time PCR. The morphological study and the molecular analysis were able to identify 11 species of Fusarium associated to insects, being the most abundant F. circinatum, F. solani, F. oxysporum and F. proliferatum. All the insect species studied showed at least one species of Fusarium, except Hylastes attenuates e Hylastes ater. Ips sexdentatus proved to be the most important insect vector, since 9 Fusarium species were detected in the specimens analyzed. |
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